Exploring trends in SARS-CoV-2 RNA concentrations in wastewater medrxivpreprint Stanford EmoryUniversity SARSCoV2 COVID19 RNA Wastewater
By Tarun Sai LomteNov 1 2022Reviewed by Aimee Molineux In a recent study posted to medRxiv* preprint server, researchers characterized trends of severe acute respiratory syndrome coronavirus 2 RNA levels in wastewater.
About the study In the present study, researchers used daily measurements of SARS-CoV-2 RNA in wastewater to compare three trend analysis methods and evaluate their performance. Daily sampling from the San José-Santa Clara regional wastewater facility commenced on November 15, 2020, until September 15, 2022. The authors used data on SARS-CoV-2 nucleocapsid gene concentrations in gene copies/g of dry weight normalized by pepper mild mottle virus concentrations.
Related StoriesHeatmaps were created for the entire analysis period and separately for three COVID-19 waves caused by 1) SARS-CoV-2 Delta, 2) Omicron BA.1, and 3) Omicron BA.2 and BA.5 variants. Further, the authors down-sampled the dataset to repeat PC and MK trend test methods. The down-sampled dataset was generated for all combinations covering frequencies between two samples/week and six samples/week.
Likewise, PC identified the increasing trend 16 and 26 days before RSI at the beginning of the Delta and BA.2/BA.5 waves, respectively. Furthermore, the MK trend test and PC identified the downward trends at the end of the COVID-19 waves before RSI. In the down-sampling analyses, both the MK trend test and PC had poor sensitivity for low sampling frequencies but improved with the increase in frequency.
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