Nature research paper: Architecture and self-assembly of the jumbo bacteriophage nuclear shell
Protein expression and purification
Full-length Chimallin from bacteriophages 201phi2-1 and Goslar were cloned with an N-terminal TEV protease-cleavable Histag using UC Berkeley Macrolab vector 2-BT . Truncations and other modified constructs were cloned by PCR mutagenesis and isothermal assembly, and inserted into the same vector. Proteins were expressed in= 0.8, inducing expression with 0.3 mM IPTG, then growing cells at 20 °C for 16–18 h.
All samples were imaged using a Titan Krios G3 transmission electron microscope operated at 300 kV configured for fringe-free illumination and equipped with a K2-directed electron detector mounted post Quantum 968 LS imaging filter . The microscope was operated in EFTEM mode with a slit-width of 20 eV and using a 70 µm objective aperture. Automated data acquisition was performed using SerialEM-v3.8b11For the 201phi2-1 24mer sample, tilt series were acquired using a pixel size of 1.
Österreich Neuesten Nachrichten, Österreich Schlagzeilen
Similar News:Sie können auch ähnliche Nachrichten wie diese lesen, die wir aus anderen Nachrichtenquellen gesammelt haben.
Human pressure drives biodiversity–multifunctionality relationships in large Neotropical wetlands - Nature Ecology & EvolutionAnalysis of the species richness and functional diversity among species across 72 lakes finds that both variables are positively associated with ecosystem multifunctionality, but that—for smaller organisms only—these positive relationships break down with increasing human pressure.
Weiterlesen »
A non-canonical vitamin K cycle is a potent ferroptosis suppressor - NatureBiochemical and lipidomic analyses identify an anti-ferroptotic function of vitamin K and reveal ferroptosis suppressor protein 1 (FSP1) as the enzyme mediating warfarin-resistant vitamin K reduction in the canonical vitamin K cycle.
Weiterlesen »
Strong yet ductile nanolamellar high-entropy alloys by additive manufacturing - NatureAn additive manufacturing strategy is used to produce dual-phase nanolamellar high-entropy alloys that show a combination of enhanced high yield strength and high tensile ductility.
Weiterlesen »
Inhibition of ASGR1 decreases lipid levels by promoting cholesterol excretion - NatureInhibiting the asialoglycoprotein receptor ASGR1 increases cholesterol excretion to the bile and then faeces, providing a unique way to lower cholesterol, and therefore providing a safe and effective way to treat cardiovascular disease.
Weiterlesen »
Brown-fat-mediated tumour suppression by cold-altered global metabolism - NatureMild cold exposure activates a substantial amount of brown adipose tissue (BAT) in a patient with cancer, reducing tumour-associated glucose uptake, and activation of BAT in mice inhibits the growth of tumours by decreasing blood glucose and impeding glycolysis-based metabolism in cancer cells.
Weiterlesen »
Modular strategy for development of the hierarchical visual network in mice - NatureThe visual network in mice develops in a modular manner with initial establishment of parallel modules followed by their concatenation.
Weiterlesen »