Inhibition of ASGR1 decreases lipid levels by promoting cholesterol excretion - Nature

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Inhibition of ASGR1 decreases lipid levels by promoting cholesterol excretion - Nature
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Nature research paper: Inhibition of ASGR1 decreases lipid levels by promoting cholesterol excretion

. Fifty-six hours after transfection, cells were switched to DMEM containing 10% FBS or cholesterol depletion medium. After 16 h, cells were harvested, lysed and subjected to immunoblotting., Huh7 cells were transfected with indicated plasmids. Thirty-two hours after transfection, cells were switched to DMEM containing 10% FBS or cholesterol depletion medium. After 16 h, cells were harvested, lysed, and subjected to immunoblotting.did not influence Dil-LDL uptake.

The internalized Dil-LDL in WT cells at 0 h is defined as 0 .did not change the PCSK9-induced LDLR degradation. Huh7 cells were transfected with indicated siRNA. The cells were incubated in DMEM containing 10% FBS or cholesterol depletion medium for 12 h. The cells were treated with indicated amounts of PCSK9 protein for additional 4 h.mice were fed an HF/HC/BS diet for 2 days. Before measuring the uptake rate, the mice were fasted for 17 h and refed for 6 h.

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