Scientists have surveyed to understand the need for clinical application of next-generation sequencing in microbiology laboratories.
By Dr. Sanchari Sinha Dutta, Ph.D.Aug 23 2023Reviewed by Sophia Coveney Scientists from Belgium have surveyed to understand clinicians’ perspectives on the need for clinical application of next-generation sequencing in microbiology laboratories. The study is published in the journal Frontiers in Medicine.
In the field of microbiology, the application of NGS is mostly limited to academic or reference laboratories. The major obstacle against its implementation in clinical microbiology is the lack of standardized protocols or tools. Survey design This online survey was conducted between January and August 2019 among clinicians practicing in hospitals located in Brussels, Belgium. The survey covered three major topics, including knowledge related to NGS, the expected diagnostic value of NGS, and the expected impact on antimicrobial prescription.
The analysis of answers provided by most of the clinicians in open fields indicated that a wide range of syndromes and samples often remain negative even if there is a strong suspicion of infection. About 83% of clinicians reported considering empirical treatment because of the lack of identification of the exact causative pathogen. Specifically, the survey findings indicated that antibiotics are prescribed blindly in most cases, followed by the prescription of corticoids and antivirals in 40% and 25% of cases, respectively.
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