Spatial and functional arrangement of Ebola virus polymerase inside phase-separated viral factories - Nature Communications

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Spatial and functional arrangement of Ebola virus polymerase inside phase-separated viral factories - Nature Communications
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A new look inside Ebola's 'viral factories' ljiresearch NatureComms

. To interrogate both hypotheses, one can follow the changes in the appearance of network-like VFs in live cells infected with fluorescence-tagged Ebola virus using light microscopy in a high-containment laboratory.

Based on our confocal light and electron microscopy findings, we propose a model in which EBOV polymerase molecules act in concert in a spatially restricted manner. Having a group of EBOV polymerase molecules acting together on the same viral genome would compensate for the low efficiency of viral RNA synthesis, as a productive viral RNA synthesis event can only initiate at a single site at the 3′ end of the viral genome.

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