Researchers have developed and demonstrated a new method for high-throughput single-cell sorting that uses stimulated Raman spectroscopy rather than the traditional approach of fluorescence-activated cell sorting. The new approach could offer a label-free, nondestructive way to sort cells for a variety of applications, including microbiology, cancer detection and cell therapy.
Jing Zhang from Boston University will present this research at Frontiers in Optics + Laser Science , which will be held 9 -- 12 October 2023 at the Greater Tacoma Convention Center in Tacoma , Washington.
Flow cytometry is used in many biomedical fields to rapidly count and characterize various types of cells, including blood cells, stem cells, cancer cells and microorganisms. Sorting cells based on their size, granularity or expression of cell surface and intracellular molecules can be used to gain insights into biological processes or to separate out cells with certain characteristics for additional analysis.
In the new work, the researchers describe how they overcame this challenge by using stimulated Raman spectroscopy, which produces a signal several orders of magnitude higher than the more commonly used spontaneous Raman scattering. For sorting, stimulated Raman images are acquired to identify objects or cells of interest, and then 2D galvo mirrors point a 532-nm pulsed laser to the cell.
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