MicroRNAs associated with diabetes in human pancreatic islets NIH MicroRNA Diabetes HumanPancreaticIslets
Background Approximately 240 loci have been found in genetic research as being related to type 2 diabetes risk, although most of these loci are located in non-coding areas, hiding the underlying molecular mechanisms. Some of the most significant insights into the molecular determinants of normal islet function and T2D pathogenesis are from recent research examining messenger RNA expression within human pancreatic islets.
The team collected 69 HPI samples and conducted RNA-sequencing , small-RNA-sequencing , and genotyping while retaining 39 specimens with RNA-seq, 63 specimens with smRNA-seq, and 57 specimens with genotypes after performing quality control. The smRNA-seq data was sourced from two different experiments: library preparation 1 and LP2.
Results Omics eBook Compilation of the top interviews, articles, and news in the last year. Download a free copy The average number of read pairs produced per sample in LP1 was 38.87 million, with an average read length of 23.24 nucleotides. On average, each LP2 sample produced 64.36 million read pairs, with a mean read length of 22.62 nucleotides. The miRNAs, such as miR-375 were also found to be the most abundant miRNAs among the islets across both LPs.
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