mAb-based bELISA developed to detect SARS-CoV-2 antibodies in a broad spectrum of animal species mSphereJ UofIllinois Cornell SARSCoV2 COVID19 coronavirus covid antibodies
By Tarun Sai LomteJul 11 2023Reviewed by Danielle Ellis, B.Sc. In a recent study published in mSphere, researchers developed a monoclonal antibody -based blocking enzyme-linked immunosorbent assay to detect severe acute respiratory syndrome coronavirus 2 in animals.
The study and findings In the present study, researchers generated a panel of anti-SARS-CoV-2 mAbs and developed a mAb-based bELISA to detect seroconversion in animals. The SARS-CoV-2 Wuhan strain nucleocapsid gene was cloned and recombinantly expressed. Mice were immunized with the recombinant protein to generate specific mAbs. Four mAbs were obtained.
Further, the authors selected the mAb, 127-3, to develop bELISA, given its strong reactivity to SARS-CoV-2 variants and inability to cross-react with common CoVs or SARS-CoV-1. They established serum standards using samples from 24 cats 14 days after SARS-CoV-2 infection; this served as the positive control. Likewise, sera from SARS-CoV-2-negative cats served as the negative control.
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