An inducible mosaic animal for perturbation (iMAP) enables in situ CRISPR targeting of 100 genes across multiple tissues in mice. NBThighlight
iMAP enables rapid derivation of single-gene perturbation mouse lines via breedingHere, we report inducible mosaic animal for perturbation , a transgenic platform enablingCRISPR targeting of at least 100 genes in parallel throughout the mouse body. iMAP combines Cre-loxP and CRISPR-Cas9 technologies and utilizes a germline-transmitted transgene carrying a large array of individually floxed, tandemly linked gRNA-coding units.
Cre-mediated recombination triggers expression of all the gRNAs in the array but only one of them per cell, converting the mice to mosaic organisms suitable for phenotypic characterization and also for high-throughput derivation of conventional single-gene perturbation lines via breeding.
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