Study suggests the brain works like a resonance chamber NatureComms
= 1 postmortem)) weighing 216 ± 30 g and aged 8.7 ± 1.6 weeks were used in this study. To minimize variability across animals, we chose all animals of the same sex. Animals were reared in a temperature-controlled room and held under a 12 h/12 h light/dark cycle with ad libitum access to food and water.
Anesthesia was induced with 5% isoflurane mixed with oxygen-enriched air in a custom-built plastic box. Rats were then weighed, moved to the animal bed and isoflurane was reduced to 2.5%. Eye ointment was applied to prevent eye dryness. In the six living rats, a 0.05 mg/kg bolus of medetomidine solution was injected subcutaneously 5–8 min after induction, immediately followed by a constant infusion of 0.1 mg/kg/h s.c. of the same solution , delivered via a syringe pump until the end of the experiment, and by a 10 min-long period where isoflurane was gradually decreased to 0%. fMRI acquisitions began once the animals stabilized in this condition . For each rat, 2 fMRI scans were first acquired under medetomidine only .
The breathing frequency and rectal temperature were monitored throughout the MRI sessions using a pillow sensor and a 9 ft optic fiber probe with a 1 mm tip , respectively, with PC-SAM 8.02 software. At the end of the experiments, medetomidine sedation was reverted by injecting 0.25 mg/kg s.c. of atipamezole .
The seventh rat, reared in the same conditions, was injected with 1 mL pentobarbital i.p. and scanned postmortem with the same MRI protocol to serve as a control.MRI data were acquired using the software ParaVision 6.0.1. Animals were imaged using a 9.4 T BioSpec® MRI scanner equipped with an AVANCEIII HD console, producing isotropic pulsed field gradients of up to 660 mT/m with a 120 µs rise time.
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