Two-organ chip developed to answer fatty liver questions CommsBio
. Briefly, the control layer consisted of microchannels supplying hydraulic pressure that were cast from a 30 µm thickness of negative photoresist mold and patterned using a standard photolithography tool. The perfusion layer consists of two cell culture chambers interconnected by semi-elliptical microchannels . The mold fabrication process for the perfusion layer followed the multilayer lithography principle, which combines standard photolithography and grayscale lithography .
To ensure the absence of mycoplasma contamination in cellular samples, a mycoplasma detection assay was conducted by using the MycoAlert™ mycoplasma detection kit according to the manufacturer’s instructions. The positive and negative controls were included in each assay run to ensure the accuracy of the results. By measuring the luminescence before and after the addition of the MycoAlert™ Substrate , a ratio was used to identify the presence or absence of mycoplasma.
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