Nature research paper: Genetic stabilization of attenuated oral vaccines against poliovirus types 1 and 3
of virus) and survival was monitored for 21 days. Mice were euthanized and marked dead when the humane endpoint was observed.Four-week-old PVRTg21/IFNR-KO mice were randomly allocated to groups for intraperitoneal injection of 100 µl of inoculum delivering various doses of Sabin 1/3 or nOPV1/3. Blinding was not applied. Blood samples were collected from the retro-orbital sinus on day 21 post inoculation for neutralization assay.
The virus–serum mixture was then transferred to another 96-well plate preseeded with HeLa S3 cells and incubated at 33 °C for 7 days. The plates were fixed with formaldehyde and stained with 0.5% crystal violet to determine cytopathic effect. Antibody titre was defined as the highest dilution sufficient to inhibit the development of cytopathic effect. Pre-immunized human serum was included as a neutralizing standard serum.
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