Efficient engineering of human and mouse primary cells using peptide-assisted genome editing
We thank M. Szurgot and R. Marmorstein for sharing the protease ULP1 expression vector and purification protocol. We also thank the staff at the Flow Cytometry Core Laboratory of Children’s Hospital of Philadelphia. G.A.B. acknowledges NIH/NHLBI . R.M.K. acknowledges NIH . E.J.W. acknowledges support from the NIH , funding from the Allen Institute for Immunology and the Parker Institute for Cancer Immunotherapy.
Zhen Zhang, Diqiu Ren, Kunhua Qin, Sierra M. Collins, Hua Huang, Chad A. Komar, Peter F. Bailer, Gerd A. Blobel, Rahul M. Kohli, Shelley L. Berger & Junwei ShiZhen Zhang, Sierra M. Collins, Hua Huang & Shelley L. Berger Department of Systems Pharmacology and Translational Therapeutics, University of Pennsylvania, Philadelphia, PA, USAInstitute for Immunology and Immune Health, University of Pennsylvania, Philadelphia, PA, USADepartment of Cancer Biology, University of Pennsylvania, Philadelphia, PA, USAAbramson Family Cancer Research Institute, University of Pennsylvania, Philadelphia, PA, USADivision of Hematology, The Children’s Hospital of Philadelphia, Philadelphia, PA, USADepartment of Medicine,...
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