CRISPRtechnology demonstrates success in preventing and treating COVID nchembio
Human embryonic kidney 293T , 293FT and Vero-ACE2 cells were grown in Dulbecco’s modified Eagle’s medium supplemented with 1% penicillin/streptomycin and 10% fetal bovine serum . Caco-2 cells were grown in DMEM supplemented with 1% penicillin/streptomycin and 20% FBS. Vero-ACE2 cells stably expressing TMPRSS2 were generated by transduction of Vero-ACE2 cells with a lentiviral vector expressing human TMPRSS2, followed by blasticidin S HCl selection for 7 d.
Primers were synthesized by IDT and their sequences are listed in Supplementary Tablemethod and the raw data was normalized by the internal controlRNA-seq analysis was carried out as previously described in ref.vectors in combination with a CasRx expression plasmid and incubated for 24 h. RNA was extracted using the RNeasy Mini Kit . For library preparation, mRNA was first enriched with NEBNext Poly mRNA Magnetic Isolation Module .
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