Nature research paper: A physical wiring diagram for the human immune system
—may indeed exist, but appears not to be sufficient to account for why certain receptors have evolved the binding kinetic rates we observe.
More broadly, the integrated approaches that we pioneered here for disentangling the immune system provide a framework for future systematic investigations. Using our high-throughput biochemical method for interaction screening and the strategies that we describe here to characterize interactions by combinations of multiplex cellular assays and genomics datasets, a range of other cellular communities in the human body could similarly be quantitatively mapped.
. Transfections for protein complexes such as integrins were done using equimolar ratios of plasmids encoding both chains, except for HLA-related complexes for which only 10% of the total plasmid encoded βmicroglobulin.
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